Chatterjee P, Stevens HY, Kippner LE, Bowles-Welch AC, Drissi H, Mautner K, Yeago C, Gibson G, Roy K. Single-cell Transcriptome and Crosstalk Analysis Reveals Immune Alterations and Key Pathways in the Bone Marrow of Knee Osteoarthritis Patients. iScience 2024 https://doi.org/10.1016/j.isci.2024.110827
Wang B, Chen RQ, Roy K. Interfacing data science with cell therapy manufacturing; where we are and where we need to be. Cytotherapy 2024 Sep https://doi.org/10.1016/j.jcyt.2024.03.011
Liu Z, Wang B, Kanwar B, Wang K, Bowles-Welch A, Balakirsky S, Roy K, ZhangC. An in-situ localized cell density monitoring method for adherent cells in hollow fiber bioreactors. Sensors and Actuators B: Chemical 2024 May https://doi.org/10.1016/j.snb.2024.135467
Chen RQ, Joffe B, Casteleiro Costa P, Filan C, Wang B, Balakirsky S, Robles F, Roy K, Li J. Real-time semantic segmentation and anomaly detection of functional images for cell therapy manufacturing. Cytotherapy 2023 Dec https://doi.org/10.1016/j.jcyt.2023.08.011
Li Y, Stevens HY, Sivaraman S, Porter LN, Hoffman AR, Gibb SL, Selvam S, Bowles-Welch AC. Streamlined Methods for Processing and Cryopreservation of Cell Therapy Products Using Automated Systems. Bio-protocol 2023 Dec 20;13(24):e4900. doi: 10.21769/BioProtoc.4900
Mautner K., Gottschalk M., Boden S. D., Akard A., Bae W. C., Black L., Boggess B., Chatterjee P., Chung C. B., Easley K. A., Gibson G., Hackel J., Jensen K., Kippner L., Kurtenbach C., Kurtzberg J., Mason R. A., Noonan B., Roy K., Valentine V., Yeago C., Drissi H. Cell-based versus corticosteroid injections for knee pain in osteoarthritis: a randomized phase 3 trial. Nat Med 29, 3120–3126 2023. https://doi.org/10.1038/s41591-023-02632-w
Van Grouw A, Colonna MB, Maughon TS, Shen X, Larey AM, Moore SG, Yeago C, Fernandez FM, Edison AS, Stice SL, Marklein RA, Bowles-Welch AC. Development of a Robust Consensus Modeling Approach for Identifying Cellular and Media Metabolites Predictive of Mesenchymal Stromal Cell Potency. Stem Cells, 2023, 41(8):792-808. doi: 10.1093/stmcls/sxad039 PMID: 37279550
Culberson AL, Bowles-Welch AC, Wang B, Kottke PA, Jimenez AC, Roy K, Fedorov AG. Early detection and metabolic pathway identification of T cell activation by in-process intracellular mass spectrometry. Cytotherapy. 2023 Sep;25(9):1006-1015. doi: 10.1016/j.jcyt.2023.03.010
Keenum MC, Chatterjee P, Atalis A, Pandey B, Jimenez A, Roy K. Single-cell epitope-transcriptomics reveal lung stromal and immune cell response kinetics to nanoparticle-delivered RIG-I and TLR4 agonists. Biomaterials. 2023 Jun;297:122097. doi: 10.1016/j.biomaterials.2023.122097
Bowles-Welch AC, Jimenez AC, Stevens HY, Frey Rubio DA, Kippner LE, Yeago C, Roy K. Mesenchymal stromal cells for bone trauma, defects, and disease: Considerations for manufacturing, clinical translation, and effective treatments. Bone Rep. 2023 Jan 20;18:101656. doi: 10.1016/j.bonr.2023.101656
Vantucci CE, Guyer T, Leguineche K, Chatterjee P, Lin A, Nash KE, Hastings MA, Fulton T, Smith CT, Maniar D, Frey Rubio DA, Peterson K, Andraca Harrer J, Willett NJ, Roy K, Guldberg RE. Systemic Immune Modulation Alters Local Bone Regeneration in a Delayed Treatment Composite Model of Non-Union Extremity Trauma. Front. Surg., 2022 07 July, Sec. Reconstructive and Plastic Surgery. Doi: 10.3389/fsurg.2022.934773
Casteleiro Costa P, Wang B, Filan C, Bowles-Welch A, Yeago C, Roy K, Robles FE. Functional imaging with dynamic quantitative oblique back-illumination microscopy. J Biomed Opt. 2022 Jun;27(6):066502. doi: 10.1117/1.JBO.27.6.066502
Jimenez AC, Heist CA, Navaei M, Yeago C, Roy K. Longitudinal two-dimensional gas chromatography mass spectrometry as a non-destructive at-line monitoring tool during cell manufacturing identifies volatile features correlative to cell product quality. Cytotherapy. 2022 Nov;24(11):1136-1147. doi: 10.1016/j.jcyt.2022.06.001
Culberson AL, Chilmonczyk MA, Kottke PA, Bowles-Welch AC, Ghoshal D, Federov AG. Sample-to-Analysis Platform for Rapid Intracellular Mass Spectrometry. Lab on a Chip, 2021 21(23):4696-4706. doi: 10.1039/d1lc00884f PMID: 34751694
Medrano-Trochez, C., Chatterjee, P., Pradhan, P. et al. Single-cell RNA-seq of out-of-thaw mesenchymal stromal cells shows tissue-of-origin differences and inter-donor cell-cycle variations. Stem Cell Res Ther 12, 565 2021. https://doi.org/10.1186/s13287-021-02627-9
Wang, B., Bowles-Welch, A. C., Yeago, C., Roy, K., Process analytical technologies in cell therapy manufacturing: State-of-the-art and future directions. J. Adv. Manuf. Process. 2021 4(1), e10106. https://doi.org/10.1002/amp2.10106
Doron G, Klontzas ME, Mantalaris A, Guldberg RE, Temenoff JS. Multiomics characterization of mesenchymal stromal cells cultured in monolayer and as aggregates. Biotechnol Bioeng 2020 Jun;117(6):1761-1778. doi: 10.1002/bit.27317.
Stevens HY, Bowles AC, Yeago C, and Roy K. Molecular Crosstalk Between Macrophages and Mesenchymal Stromal Cells. Front. Cell Dev. Biol. 2020 8:600160. doi: 10.3389/fcell.2020.600160